The Czech Republic has emerged as a vibrant hub for innovation, with its startup ecosystem gaining global recognition. From Prague to Brno, startups are making strides in Artificial Intelligence, fintech, green energy, and health tech. Here are ten of the fastest-growing Czech startups in 2025.
1. Rezolv Energy
- Sector: Renewable Energy
- Location: Prague
- Funding: €90 million
Rezolv Energy is revolutionizing renewable energy with wind, solar, and battery storage solutions. By providing subsidy-free, stable-priced energy for industrial and commercial users, it is becoming a leader in sustainable power across Central and Eastern Europe.
2. Kardi AI
- Sector: Health Tech
- Location: Olomouc
- Funding: €1.9 million
Kardi AI combines wearable trackers with AI to monitor cardiovascular health remotely. Its innovative approach ensures early detection and continuous monitoring for users outside medical facilities.
3. CRUXO
- Sector: AdTech and E-commerce
- Location: Prague
- Funding: €800k
CRUXO helps retailers optimize advertising through real-time data insights. Its platform boosts shopper engagement and revenue by automating targeted ads, making it a game-changer for sectors like beauty, electronics, and groceries.
4. Flowlance
- Sector: Creator Economy
- Location: Prague
- Funding: €500k
Flowlance empowers creators to monetize their knowledge by offering tools to sell services, manage bookings, and track sales—all on a user-friendly platform that requires no technical expertise.
5. RobosizeME
- Sector: Automation as a Service (AaaS)
- Location: Olomouc
- Funding: €1 million
RobosizeME automates operational tasks in the hospitality industry, such as rate loading and payment reconciliation. This allows hotel staff to focus on enhancing guest experiences.
6. Better Stack
- Sector: Developer Tools and Monitoring
- Location: Prague
Better Stack is gaining traction as an all-in-one monitoring platform for developers. It integrates incident management with real-time data visualization to help teams maintain system reliability.
7. Blindspot Technologies
- Sector: Cybersecurity
- Location: Prague
- Funding: $1.53 million
Blindspot Technologies focuses on advanced DDoS protection for businesses. Its innovative cybersecurity solutions are becoming essential in the era of increasing digital threats.
8. Superface
- Sector: AI-Powered Business Integration
- Location: Prague
- Funding: $4.41 million
Superface uses self-integration technology to replace traditional APIs, enabling seamless business integrations without human intervention.
9. Hedepy
- Sector: Health Tech (Mental Health)
- Location: Prague
- Funding: $5.4 million
Hedepy provides accessible psychotherapy through video calls, breaking barriers to mental health care with its user-friendly platform.
10. Twisto
- Sector: FinTech
- Location: Prague
- Funding: $21.83 million (Series C)
Twisto simplifies online payments by offering cash flow management tools and one-click payment solutions for consumers, making it a leader in the fintech space.
The Czech Startup Ecosystem in 2025
The Czech Republic is now recognized as the “Silicon Valley of Central Europe,” driven by its talented STEM workforce (science, technology, engineering, and mathematics) and government-backed initiatives like startup visas. With over 700 startups in Prague alone and increasing venture capital interest, the country is set to remain a critical player in global innovation.
Damalion supports entrepreneurs to launch their sro (Společnost s ručením omezeným) or limited liability company in Czech Republic. Please contact your Damalion expert now.
This information is not intended to be a substitute for specific individualized tax or legal advice. We suggest that you discuss your specific situation with a qualified tax or legal advisor.
This information includes the introduction of contracted clients to accredited banks and private debt lenders. Clients are free to use our services or enter into an agreement with the said accredited lenders.
The information provided is for informational purposes only and does not constitute an offer or solicitation to buy or sell shares or securities of any type of investment vehicle. The content herein is not intended to serve as the basis for any investment decision, nor does it include specific investment recommendations. As such, this document does not constitute investment advice, counsel, or a solicitation to invest in any security. It should not be interpreted as an offer to sell, a subscription invitation, or a request to purchase or subscribe to any securities. Furthermore, no part of this document should form the foundation of or be relied upon in connection with any agreement, contract, or commitment of any kind. Damalion explicitly disclaims all liability for any direct or indirect loss or damage arising from: (i) reliance on the information provided herein, (ii) errors, omissions, or inaccuracies within this information, or (iii) actions taken based on this information.
